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Anti-CDC6 PHOSPHO Antibody EPR759Y
Also for CDC6 (NM_001254)
|A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 54.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:2000; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens cell division cycle 6 (CDC6)|
|CDC18L; HsCDC18; HsCDC6|
|The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6p and cdc18+ are unstable proteins that are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. They participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). Cdc6 is believed to be phosphorylated at Serine 54 during S phase and functions as a chromatin-bound signal that prevents reformation of prereplication complexes (4). |
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Western blot - Cdc6 (phospho S54) antibody [EPR759Y]; All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] at 1/2000 dilution.Lane 1 : Raji cell lysate.Lane 2 : Raji + FBS cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 63 kDa.Observed band size : 63 kDa.
Immunocytochemistry/ Immunofluorescence - Cdc6 (phospho S54) antibody [EPR759Y]; Immunofluorescent staining of Cdc6 in HeLa cells using ab75809 at 1/100 dilution.