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Anti-SMC1A PHOSPHO Antibody EP2858Y


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against SMC1A (Clone EP2858Y) (Phospho-specific)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding serine 966 of human SMC1 was used as an immunogen. This antibody detects SMC1 phosphorylated on Serine 966.
Clone NameEP2858Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:5000 - 1:10000; ICC/IF: 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens structural maintenance of chromosomes 1A (SMC1A), transcript variant 1
Alternative NameCDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB
Database LinkNP_006297
Entrez Gene 8243 Human
FunctionStructural maintenance of chromosome proteins (SMC) are evolutionarily conserved chromosomal proteins that are components of the cohesin complex, necessary for sister chromatid cohesion. These proteins may also function in DNA repair. It has been reported that SMC1 is a component of the DNA damage response network that functions as an effector in the ATM/NBS1-dependent S-phase checkpoint pathway. SMC1 associates with BRCA1 and is phosphorylated in response to IR in an ATM- and NBS1-dependent manner (1). ATM phosphorylates Smc1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint. Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM substrates Nbs1 and Brca1 (2).
Related PathwayDruggable Genome Cell cycleOocyte meiosis

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Western blot - SMC1 (phospho S966) antibody [EP2858Y]; All lanes : Anti-SMC1 (phospho S966) antibody [EP2858Y] at 1/1000 dilution.Lane 1 : HeLa cell lysates un-treated.Lane 2 : HeLa cell lysates treated with hydroxyurea.Lysates/proteins at 10 ug per lane.Secondary.HRP goat anti-rabbit at 1/2000 dilution.Predicted band size : 143 kDa.Observed band size : 160 kDa .
Immunocytochemistry/ Immunofluorescence - SMC1 (phospho S966) antibody [EP2858Y]; Immunofluorescent staining of HeLa cells using TA301252 at a 1/250 dilution.


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