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Anti-SMC1A PHOSPHO Antibody EP2858Y
Also for SMC1A (NM_006306)
|A phospho specific peptide corresponding to residues surrounding serine 966 of human SMC1 was used as an immunogen. This antibody detects SMC1 phosphorylated on Serine 966.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000 - 1:10000; ICC/IF: 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens structural maintenance of chromosomes 1A (SMC1A), transcript variant 1|
|CDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB|
|Structural maintenance of chromosome proteins (SMC) are evolutionarily conserved chromosomal proteins that are components of the cohesin complex, necessary for sister chromatid cohesion. These proteins may also function in DNA repair. It has been reported that SMC1 is a component of the DNA damage response network that functions as an effector in the ATM/NBS1-dependent S-phase checkpoint pathway. SMC1 associates with BRCA1 and is phosphorylated in response to IR in an ATM- and NBS1-dependent manner (1). ATM phosphorylates Smc1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint. Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM substrates Nbs1 and Brca1 (2). |
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Western blot - SMC1 (phospho S966) antibody [EP2858Y]; All lanes : Anti-SMC1 (phospho S966) antibody [EP2858Y] at 1/1000 dilution.Lane 1 : HeLa cell lysates un-treated.Lane 2 : HeLa cell lysates treated with hydroxyurea.Lysates/proteins at 10 µg per lane.Secondary.HRP goat anti-rabbit at 1/2000 dilution.Predicted band size : 143 kDa.Observed band size : 160 kDa .
Immunocytochemistry/ Immunofluorescence - SMC1 (phospho S966) antibody [EP2858Y]; Immunofluorescent staining of HeLa cells using ab81306 at a 1/250 dilution.