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Anti-EIF4EBP1 PHOSPHO Antibody EPR729(2)Y
Also for EIF4EBP1 (NM_004095)
|A phospho specific peptide corresponding to residues surrounding threonine 37 was used as an immunogen. This antibody detects 4E-BP1 phosophorylated at threonine 37.|
||Tissue culture supernatant
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:2000
|Does not react with Rat. Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
|Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1)|
|4E-BP1; 4EBP1; BP-1; PHAS-I|
Entrez Gene 1978 Human
Entrez Gene 13685 Mouse
|4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eIF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP1. Non-phosphorylated 4E-BP1 will bind strongly to eIF4E while, the phosphorylated form will no (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 70 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3). It has been reported previously that phosphorylation of 4E-BP1 on Thr 37 and Thr 46 is relatively insensitive to serum deprivation and rapamycin treatment, and that phosphorylation of these residues is required for the subsequent phosphorylation of a set of unidentified serum-responsive sites (4) |
| ErbB signaling pathwaymTOR signaling pathwayInsulin signaling pathwayAcute myeloid leukemia|
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Western blot - eIF4EBP1 (phospho T37) antibody [EPR729(2)Y]; All lanes : Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] at 1/2000 dilution.Lane 1 : 293 cell lysate, untreated.Lane 2 : 293 cell lysate, treated with insulin.Lysates/proteins at 10 ug per lane.Secondary.HRP-conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 13 kDa.Observed band size : 17 kDa .