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Anti-PARK7 Antibody EP2815Y
Also for PARK7 (NM_007262)
|A synthetic peptide corresponding to residues near N-terminus of human DJ-1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:10000 - 1:20000; IP: 1:20; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; FC: 1:100; ICC/IF: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens parkinson protein 7 (PARK7), transcript variant 1|
|DJ-1 is ubiquitously expressed in various human tissues, and expression is induced by growth stimuli. Moreover, DJ-1 translocates from cytoplasm to nuclei in the S phase of the cell cycle. DJ-1 is thus suggested to be a novel mitogen-dependent oncogene product involved in a Ras-related signal transduction pathway (1). DJ-1 was first identified as a novel candidate of the oncogene product that transformed mouse NIH3T3 cells in cooperation with an activated ras. Later DJ-1 was also found to be an infertility-related protein that was reduced in rat sperm treated with sperm toxicants that cause infertility in rats. Results of further tests indicate that DJ-1 is a positive regulator of the androgen receptor (2). Mutations in a gene on chromosome 1, DJ-1, have been reported recently to be associated with recessive, earlyonset Parkinson's disease. The L166P mutation has the simple effect of promoting DJ-1 degradation, thereby reducing net DJ-1 protein within the cell (3). |
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Western blot - PARK7/DJ1 antibody [EP2815Y]; All lanes : Anti-PARK7/DJ1 antibody [EP2815Y] at 1/20000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : NIH3T3 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 20 kDa.Observed band size : 23 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARK7/DJ1 antibody [EP2815Y]; ab76008, at 1/250 dilution, staining PARK7/DJ1 in human brain by immunohistochemistry using paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EP2815Y]; ICC/IF image of ab76008 stainedPANC-1cells. The cells were 4%formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normalgoat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2815Y]; Overlay histogram showing HepG2 cells stained with ab76008 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.