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Anti-EPS15L1 Antibody EP1146Y
Also for EPS15L1 (NM_021235)
|A synthetic peptide corresponding to residues near the N-terminus of human EPS15R was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:20000; FC: 1:20; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens epidermal growth factor receptor pathway substrate 15-like 1 (EPS15L1), transcript variant 2|
|EPS15 and EPS15R are substrates of the epidermal growth factor (EGF) receptor kinase that are characterized by the presence of a protein:protein interaction domain, the EH domain, and by their ability to bind to the clathrin adaptor protein complex adaptor protein 2. Evidence suggests that eps15 and eps15R are involved in endocytosis (1). The most striking difference between these two related proteins is that Eps15R is also found in the nucleus, whereas Eps15 is excluded from this compartment at steady state (2). Eps15R plays a key role in clathrin-mediated endocytosis of transmembrane receptors. Using a mutational approach, it has been found that the second ubiquitin-interacting motif (UIM) of Eps15 and Eps15R is essential for their ubiquitination (3). |
EGFR1 Signaling Pathway
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Western blot - EPS15R antibody [EP1146Y]; All lanes : Anti-EPS15R antibody [EP1146Y] at 1/20000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : 293T cell lysates.Lane 3 : SHSY5Y cell lysates.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 94 kDa.Observed band size : 76,108,125 kDa .
Immunocytochemistry/ Immunofluorescence - EPS15R antibody [EP1146Y]; ab76004 at 1/100-1/250 dilution staining EPS15R in A431 cells by Immunofluorescence.