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Anti-PRKAR2B Antibody EP2649Y
Also for PRKAR2B (NM_002736)
|A synthetic peptide corresponding to residues near the C-terminus of human PKA RIIb was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:25,000~50,000; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:80; IP: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens protein kinase, cAMP-dependent, regulatory, type II, beta (PRKAR2B)|
|Cyclic AMP is an important second messenger in the coordinated regulation of cellular metabolism. Its effects are mediated by cAMP-dependent protein kinase (PKA), which is assembled from two regulatory (R) and two catalytic (C) subunits. The RIIb isoform is abundant in brown and white adipose tissue and brain, with limited expression elsewhere. Results demonstrate a role for the RIIb holoenzyme in regulating energy balance and adiposity (1). Disruption of the RIIb regulatory subunit gene of PKA results in release of the active catalytic subunit and an increase in basal PKA activity in cells where RIIb is highly expressed (2). The cAMP-dependent protein kinase system is believed to mediate most, if not all, cellular effects of the second messenger cAMP. Free, activated C subunits of PKA RIIb catalyze the phosphorylation of specific substrate proteins on serine and threonine residues and thereby alter their activity or function (3). |
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Western blot - PKA 2 beta (regulatory subunit) antibody [EP2649Y]; Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] at 1/50000 dilution + Human brain lysate at 10 µg.Secondary.goat anti rabbit HRP at 1/2000 dilution.Predicted band size : 46 kDa.Observed band size : 46 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKA 2 beta (regulatory subunit) antibody [EP2649Y]; ab75993, at a 1/100 dilution, staining PKA 2 beta (regulatory subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.
Flow Cytometry-Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y](ab75993); Overlay histogram showing SH-SY5Y cells stained with ab75993 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.