OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PRKAR2B antibodies

Anti-PRKAR2B Antibody EP2649Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301234
  • Rabbit Monoclonal Antibody against PRKAR2B (Clone EP2649Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419138) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)
spacer
Also for PRKAR2B (NM_002736)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human PKA RIIb was used as an immunogen.
Clone NameEP2649Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:25,000~50,000; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:80; IP: 1:60
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein kinase, cAMP-dependent, regulatory, type II, beta (PRKAR2B)
Alternative NamePRKAR2; RII-BETA
Database LinkNP_002727
FunctionCyclic AMP is an important second messenger in the coordinated regulation of cellular metabolism. Its effects are mediated by cAMP-dependent protein kinase (PKA), which is assembled from two regulatory (R) and two catalytic (C) subunits. The RIIb isoform is abundant in brown and white adipose tissue and brain, with limited expression elsewhere. Results demonstrate a role for the RIIb holoenzyme in regulating energy balance and adiposity (1). Disruption of the RIIb regulatory subunit gene of PKA results in release of the active catalytic subunit and an increase in basal PKA activity in cells where RIIb is highly expressed (2). The cAMP-dependent protein kinase system is believed to mediate most, if not all, cellular effects of the second messenger cAMP. Free, activated C subunits of PKA RIIb catalyze the phosphorylation of specific substrate proteins on serine and threonine residues and thereby alter their activity or function (3).
Related Pathway
Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PKA 2 beta (regulatory subunit) antibody [EP2649Y]; Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] at 1/50000 dilution + Human brain lysate at 10 ug.Secondary.goat anti rabbit HRP at 1/2000 dilution.Predicted band size : 46 kDa.Observed band size : 46 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKA 2 beta (regulatory subunit) antibody [EP2649Y]; TA301234, at a 1/100 dilution, staining PKA 2 beta (regulatory subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.
FC Image
Flow Cytometry-Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y](TA301234); Overlay histogram showing SH-SY5Y cells stained with TA301234 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title