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Anti-VAMP8 Antibody EP2629Y
Also for VAMP8 (NM_003761)
|A synthetic peptide corresponding to residues near the N-terminus of human VAMP8 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 10,000~20,000; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:80; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens vesicle-associated membrane protein 8 (VAMP8)|
|Synaptobrevins/vesicle-associated membrane proteins (VAMPs) together with syntaxins and a synaptosome-associated protein of 25 kDa (SNAP-25) are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (1). The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. It has been shown that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP8, specifically localize to the midbody during cytokinesis in mammalian cells (2). It has been established that VAMP8/endobrevin is a major player in regulated exocytosis of the exocrine pancreas. VAMP8 is enriched on the membrane of zymogen granules and exists in a complex with syntaxin 4 and SNAP-23. Results suggest a major physiological role of VAMP8 in regulated exocytosis of pancreatic acinar cells by serving as a v-SNARE of zymogen granules (3). |
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Western blot - VAMP8 antibody [EP2629Y]; All lanes : Anti-VAMP8 antibody [EP2629Y] at 1/20000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : PC12 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 17 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAMP8 antibody [EP2629Y]; ab76021, at a 1/100 dilution, staining VAMP8 in paraffin embedded human brain tissue by Immunohistochemistry. An HRP/AP polymerized secondary antibody was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP8 antibody [EP2629Y]; Immunohistochemical analysis of Human lacrimal gland tissue, staining VAMP8 with ab76021. Antigen retrieval was performed using antigen retrieval solution in a microwave. Sections were blocked with 10 goat serum for 30 minutes and incubated with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB.