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Anti-CTNNB1 PHOSPHO Antibody EP1905Y
Also for CTNNB1 (NM_001904)
|A phospho specific peptide corresponding to residues surrounding threonine 41 and 45 of human Beta-catenin was used as an immunogen. This antibody detects Beta-catenin phosphorylated on threonine 41 and/or 45.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:20000; IP: 1:20
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
|Homo sapiens catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1|
|armadillo; CTNNB; MRD19|
|Beta-catenin is a key regulatory protein involved in cell adhesion and signal transduction through the Wnt pathway, and plays important roles in development, cellular proliferation, and differentiation (1). Beta-catenin is phosphorylated at four serine and threonine residues at the N-terminus (2). Ser 45 is phosphorylated by CK1, and is thought to be a priming site for the phosphorylation by glycogen synthase kinase-3 (GSK-3) of the remaining three residues, Thr 41, Ser 37, and Ser 33 (2). Wnt signaling antagonizes the action of GSK-3, which in subsequent phosphorylation steps, leads to its degradation (3). Mutations in the -catenin gene, CTNNB1, have been implicated in its accumulation and various forms of carcinomas (4). |
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - beta Catenin (phospho T41 + T45) antibody [EP1905Y]; All lanes : Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] at 1/20000 dilution.Lane 1 : 293T cell lysates un-treated.Lane 2 : 293T cell lysates treated with calyculin A.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 86 kDa.Observed band size : 70 kDa .