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Anti-MAPT Antibody EP2456Y
Also for MAPT (NM_005910)
|A synthetic phospho-peptide corresponding to residues surrounding serine 622 of human Tau was used as immunogen. This antibody detects Tau phospholyated and unphospholyated on serine 622.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||ICC/IF: 1:100 - 1:500; WB: 1:2000 - 1:10000; FC: 1:160 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens microtubule-associated protein tau (MAPT), transcript variant 2|
|DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; TAU|
|Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Taus association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4). |
MAPK signaling pathway
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Western blot - Tau antibody [EP2456Y]; Anti-Tau antibody [EP2456Y] at 1/500000 dilution + human brain tissue lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/1000 dilution.developed using the ECL technique.Observed band size : 50 kDa .
Immunocytochemistry/ Immunofluorescence - Tau (phospho S622) antibody [EP2456Y]; ab76128 at 1/100 dilution staining Tau in SH-SY5Y cells.
Flow Cytometry - Anti-Tau antibody [EP2456Y]; Overlay histogram showing SH-SY5Y cells stained with ab76128 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.