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Anti-CAT Antibody EPR1928Y
|A synthetic peptide corresponding to residues near the N-terminus of human Catalase was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500 - 1:1000; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens catalase (CAT)|
|Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1. 5 A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erythrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflammation) (2). |
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Western blot - Catalase antibody [EPR1928Y]; All lanes : Anti-Catalase antibody [EPR1928Y] at 1/1000 dilution.Lane 1 : TF cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 60 kDa.Observed band size : 60 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Catalase antibody [EPR1928Y]; ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue.