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Home Antibody All anti-KRT7 antibodies

Anti-KRT7 Antibody EPR1619Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301161
  • Rabbit Monoclonal Antibody against KRT7 (Clone EPR1619Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401707) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(7)
IF(1)
FC(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-term of human CK-7 was used as an immunogen.
Clone NameEPR1619Y IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsWB: 1:5000 - 1:10000; IP: 1:30; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:30
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens keratin 7 (KRT7)
Alternative NameCK7; K2C7; K7; SCL
Database LinkNP_005547
Entrez Gene 3855 Human
FunctionKeratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Keratin 7 (CK7) is expressed in a wide range of epithelial structures in humans. The expression pattern in mice is similar to the reported human distribution, with substantial expression observed in lung, bladder, mesothelium, hair follicle, and ductal structures (1). Protein expression levels of CK7 appear significantly higher in chromophobe RCC compared to oncocytoma (p < 0.001). Studies provide further evidence that CK7 and parvalbumin immunostains may be useful in differentiating oncocytoma from chromophobe RCC in problematic cases (2). Also, the pattern of immunohistochemical expression of CK 7 along with CK 20 is commonly used to assess possible primary sites of metastatic carcinomas (3).
Related PathwayES Cell Differentiation/IPS

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cytokeratin 7 antibody [EPR1619Y]; All lanes : Anti-Cytokeratin 7 [EPR1619Y] antibody at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : T47D cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
Assay Image
Other-Anti-Cytokeratin 7 antibody [EPR1619Y](TA301161); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 7 antibody [EPR1619Y]; Human ovarian carcinoma tissue stained with TA301161 at 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 7 antibody [EPR1619Y](TA301161); TA301161 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 7 antibody [EPR1619Y](TA301161); TA301161 showing positive staining in Endometrial carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 7 antibody [EPR1619Y](TA301161); TA301161 showing positive staining in Ovarian carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 7 antibody [EPR1619Y](TA301161); TA301161 showing negative staining in Sarcoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 7 antibody [EPR1619Y](TA301161); TA301161 showing negative staining in Colonic adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 7 antibody [EPR1619Y](TA301161); TA301161 showing positive staining in Urinary bladder transitional carcinoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Cytokeratin 7 antibody [EPR1619Y]; HeLa cells stained with TA301161 at 1/00 dilution.
FC Image
Flow Cytometry-Anti-Cytokeratin 7 antibody [EPR1619Y](TA301161); Overlay histogram showing HeLa cells stained with TA301161 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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