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Anti-ITGB1 Antibody EPR1040Y
Also for ITGB1 (NM_002211)
|A synthetic peptide corresponding to the residues in human Integrin beta-1 (CD29) was used as immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||IHC-P: 1:100 - 1:250; FC: 1:100 - 1:1000; WB: 1:500
|Does not react with Mouse, Rat. Is unsuitable for ICC/IF or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for ICC/IF or IP.
|Homo sapiens integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A|
|CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB|
|Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4). |
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Western blot - Anti-Integrin beta 1 antibody [EPR1040Y]; All lanes : Anti-Integrin beta 1 antibody [EPR1040Y] at 1/500 dilution.Lane 1 : U937 cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 88 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR1040Y]; Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labelling Integrin beta 1 with ab134179 antibody at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR1040Y]; Immunohistochemical analysis of paraffin embedded Human brain (endothelial cells) tissue labelling Integrin beta 1 with ab134179 antibody at a dilution of 1/100.
Flow Cytometry - Anti-Integrin beta 1 antibody [EPR1040Y]; Overlay histogram showing MCF7 cells stained with ab134179 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.