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Anti-EIF2AK2 PHOSPHO Antibody EPR2152Y
Also for EIF2AK2 (NM_002759)
|A synthetic phospho-peptide corresponding to residues surrounding Thr451 of human PKR (Interferon-inducible RNA-dependent protein kinase) was used as immunogen. The antibody only detects PKR phosphorylated on Threonine 446.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: Use at an assay dependent dilution
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
|Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), transcript variant 1|
|EIF2AK1; PKR; PPP1R83; PRKR|
|Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the ?-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2?), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3). |
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Western blot - PKR (phospho T451) antibody [EPR2152Y]; All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate un-treated.Lane 2 : HeLa cell lysate treated with IFN-alpha.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 62 kDa.Observed band size : 68 kDa .