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Home Antibody All anti-CD247 antibodies

Anti-CD247 PHOSPHO Antibody EP265(2)Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301138
  • Rabbit Monoclonal Antibody against CD247 (Clone EP265(2)Y) (Phospho-specific)
100ul 325 In Stock
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WB(1)
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Also for CD247 (NM_000734)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding tyrosine 142 of human CD3 zeta was used as an immunogen. This antibody detects CD3 zeta phosphorylated on tyrosine 142.
Clone NameEP265(2)Y IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:500; ICC: 1:250; IP: 1:50
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens CD247 molecule (CD247), transcript variant 2
Alternative NameCD3-ZETA; CD3H; CD3Q; CD3Z; T3Z; TCRZ
Database LinkNP_000725
FunctionCD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways (3). Loss of the zeta chain results in the synthesis of unstable TCRs (4). The reversible phosphorylation of tyrosine residues is an important mechanism for modulating biological processes such as cellular signaling, differentiation, and growth, and if deregulated, can result in various types of cancer (5).
Related Pathway

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WB Image
Western blot - CD3 zeta (phospho Y142) antibody [EP265(2)Y]; All lanes : Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] at 1/2000 dilution.Lane 1 : Jurkat cell lysates, membrane un-treated.Lane 2 : Jurkat cell lysates, membrane treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 19 kDa.Observed band size : 19 kDa.

 

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