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Anti-TUBB3 Antibody EPR1568Y
Also for TUBB3 (NM_006086)
|A synthetic peptide corresponding to residues near the N-term of class III Beta-Tubulin was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:20000 - 1:100000; IP: 1:40; IHC-P: 1:250 - 1:500; FC: 1:1000
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens tubulin, beta 3 class III (TUBB3), transcript variant 1|
|beta-4; CDCBM; CDCBM1; CFEOM3A; TUBB4|
|Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors (3). |
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Western blot - beta III Tubulin antibody [EPR1568Y]; Anti-beta III Tubulin antibody [EPR1568Y] at 1/50000 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta III Tubulin antibody [EPR1568Y]; Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68193 at 1/250-1/500 dilution.
Flow Cytometry - Anti-beta III Tubulin antibody [EPR1568Y]; Overlay histogram showing HeLa cells stained with ab68193 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.