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Anti-LAT Antibody EP983(2)Y
|A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 200 of human LAT (long isoform) was used as immunogen. The antibody only detects LAT phosphorylated on Tyrosine 200 of long isoform and Tyrosine 171 of short isoform.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000; ICC: 1: 100~250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens linker for activation of T cells (LAT), transcript variant 1|
|LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 (on the short isoform of LAT) are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1. Tyr200 and Tyr220 on long isoform of LAT correspond to Tyr171 and Tyr191 on short isoform respectively (3). |
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Western blot - LAT (phospho Y200) antibody [EP983(2)Y]; All lanes : Anti-LAT (phospho Y200) antibody [EP983(2)Y] at 1/10000 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate, treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : ~35 kDa .