CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
Immunogen
A phospho – specific peptide corresponding to residues surrounding tyrosine 877 of human HER2 was used as an immungen. This antibody detects HER2 phosphorylated at tyrosine 877.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
HER2 / ErbB2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Autophosphorylation did not modulate receptor turnover. A Tyr----Phe substitution of ErbB-2 Tyr-877 homologous to pp60c-src Tyr-416 did not alter ErbB-2 biological and biochemical properties, thus excluding the possibility that phosphorylation of this residue, located in the kinase domain, modulates ErbB-2 gp185 catalytic function (4).
Related Pathway
Adherens junction
Focal Adhesion
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Western blot analysis on (A,B) HeLa cell lysates or (C,D) SKBR-3 using anti-Phospho-HER2 (pY877), 1:1000 dilution. Cells were either (A,C) untreated (B,D) treated with EGF
