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Home Antibody All anti-ERBB2 antibodies

Anti-ERBB2 PHOSPHO Antibody EPR2324Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301126
  • Rabbit Monoclonal Antibody against ERBB2 (Clone EPR2324Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
IF(1)
Assay(1)
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Also for ERBB2 (NM_004448)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho – specific peptide corresponding to residues surrounding tyrosine 877 of human HER2 was used as an immungen. This antibody detects HER2 phosphorylated at tyrosine 877.
Clone NameEPR2324Y IsotypeTissue culture supernatant
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IF Suggested DilutionsWB: 1:500 - 1:1000; ICC/IF: 1:50 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1
Alternative NameCD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
Database LinkNP_004439
FunctionHER2 / ErbB2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Autophosphorylation did not modulate receptor turnover. A Tyr----Phe substitution of ErbB-2 Tyr-877 homologous to pp60c-src Tyr-416 did not alter ErbB-2 biological and biochemical properties, thus excluding the possibility that phosphorylation of this residue, located in the kinase domain, modulates ErbB-2 gp185 catalytic function (4).
Related Pathway
Adherens junction
Focal Adhesion

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WB Image
Western blot - ErbB 2 (phospho Y877) antibody [EPR2324Y]; All lanes : Anti-ErbB 2 (phospho Y877) antibody [EPR2324Y] at 1/1000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with EGF.Lane 3 : SKBR3 cell lysate, untreated.Lane 4 : SKBR3 cell lysate, treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 138 kDa.Observed band size : 180 kDa .
Assay Image
Other-Anti-ErbB 2 (phospho Y877) antibody [EPR2324Y](TA301126); Equilibrium disassociation constant (KD)..
IF Image
Immunocytochemistry/ Immunofluorescence - ErbB 2 (phospho Y877) antibody [EPR2324Y]; TA301126 at 1/50 dilution staining ErbB 2 in SKBR3 cells by Immunofluorescence.

 

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