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Anti-ERBB2 PHOSPHO Antibody EPR2324Y
Also for ERBB2 (NM_004448)
|A phospho – specific peptide corresponding to residues surrounding tyrosine 877 of human HER2 was used as an immungen. This antibody detects HER2 phosphorylated at tyrosine 877.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IF
||WB: 1:500 - 1:1000; ICC/IF: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1|
|CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1|
|HER2 / ErbB2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Autophosphorylation did not modulate receptor turnover. A Tyr----Phe substitution of ErbB-2 Tyr-877 homologous to pp60c-src Tyr-416 did not alter ErbB-2 biological and biochemical properties, thus excluding the possibility that phosphorylation of this residue, located in the kinase domain, modulates ErbB-2 gp185 catalytic function (4). |
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Western blot - ErbB 2 (phospho Y877) antibody [EPR2324Y]; All lanes : Anti-ErbB 2 (phospho Y877) antibody [EPR2324Y] at 1/1000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with EGF.Lane 3 : SKBR3 cell lysate, untreated.Lane 4 : SKBR3 cell lysate, treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 138 kDa.Observed band size : 180 kDa .
Other-Anti-ErbB 2 (phospho Y877) antibody [EPR2324Y](ab75873); Equilibrium disassociation constant (KD)..
Immunocytochemistry/ Immunofluorescence - ErbB 2 (phospho Y877) antibody [EPR2324Y]; ab75873 at 1/50 dilution staining ErbB 2 in SKBR3 cells by Immunofluorescence.