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Home Antibody All anti-NTRK1 antibodies

Anti-NTRK1 Antibody EP1058Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA301109 Rabbit Monoclonal Antibody against NTRK1 (Clone EP1058Y) 100ul $325 2 Weeks
LC419273 NTRK1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for NTRK1 (NM_002529)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding tyrosine 791 of human Trk A was used as an immunogen. This antibody detects both phosphorylated and unphosphorylated Trk A.
Clone NameEP1058Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; ICC: Use at an assay dependent concentration; WB: 1:2500 - 1:5000; IP: 1:50; IHC-P: 1:100 - 1:250; FC: 1:50 - 1:100
Predicted MW Explanation 87 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 2
Alternative NameMTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA
Database LinkNP_002520
Entrez Gene 4914 Human
Entrez Gene 18211 Mouse
Entrez Gene 59109 Rat
FunctionThe Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).
Related PathwayProtein KinaseTransmembraneDruggable Genome MAPK signaling pathwayEndocytosisApoptosisNeurotrophin signaling pathwayPathways in cancerThyroid cancerMore Pathways >>

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WB Image
Western blot - TrkA antibody [EP1058Y]; All lanes : Anti-TrkA antibody [EP1058Y] at 1/5000 dilution.Lane 1 : Rat brain cell lysate un-treated.Lane 2 : Rat brain cell lysate treated with AP.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 87 kDa.Observed band size : 145 kDa .

WB Image
Western blot - Anti-TrkA antibody; All lanes : Anti-TrkA antibodyat 1/2500 dilution.Lane 1 : Human neuronal cell lysate (untreated).Lane 2 : Human neuronal cell lysate (treated with rh-NGF at 25ng/ml).Lane 3 : Human neuronal cell lysate (treated with rh-NGF at 50ng/ml).Lane 4 : Human neuronal cell lysate (treated with rh-NT3 at 50ng/ml).Lane 5 : Human neuronal cell lysate (treated with rh-BDNF 50ng/ml).Lane 6 : TrkA protein.Lysates/proteins at 15 ug per lane.

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TrkA antibody [EP1058Y]; Immunohistochemical analysis of TrkA in paraffin embedded human brain tissue using TA301109 at a 1/100 dilution.

IHC Image
IHC - Anti-TrkA antibody; TA301109 staining TrkA in murine brain tissue by IHC . .Tissue was fixed with formaldehyde, permeabilized with 0.1% Saponin/PBS and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/150 in blocking buffer) for 16 hours at 4°C. A FITC-conjugated goat anti-rabbit polyclonal IgG 1:100 was used as the secondary antibody.

IF Image
Immunocytochemistry/ Immunofluorescence - TrkA antibody [EP1058Y]; Immunofluorescent staining of TrkA in SHSY5Y cells using TA301109 at a 1/100 dilution.

IF Image
ICC/IF image of TrkA staining on culture of mouse DRG neurons using TA301109 (1:100). The cells were fixed using formaldehyde and permeabilized using 0.2% Triton X-100. The cells were blocked using 10% Goat serumfor 1 hour at 22°C.TA301109 was diluted 1:100 using PBS and incubated with the cells for 30 mins at 22°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to Alexa Fluor 488 (1:1000). Neuron was stained using Beta III tubulin antibody (Alexa Fluor 647)

FC Image
Flow Cytometry - TrkA antibody; Overlay histogram showing SH-SY5Y cells stained with TA301109 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in SH-SY5Y cells under the same conditions.


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