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Anti-STAT3 Antibody EP2147Y
Also for STAT3 (NM_213662)
|A synthetic phospho-peptide corresponding to residues surrounding tyrosine 727 of human Stat3 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:20000; IP: 1:40; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 3|
|Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4) |
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
TGF Beta Signaling Pathway
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Western blot - STAT3 (phospho Y705) antibody [EP2147Y]; All lanes : Anti-STAT3 (phospho Y705) antibody [EP2147Y] at 1/20000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate, treated with alpha interferon.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit-HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 88 kDa.Observed band size : 75 kDa .
Western blot - Anti-STAT3 (phospho Y705) antibody [EP2147Y]; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 88 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT3 (phospho Y705) antibody [EP2147Y]; Human colon carcinoma tissue stained with ab76315 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - STAT3 (phospho Y705) antibody [EP2147Y]; HeLa cells with ab76315 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence-Anti-STAT3 (phospho Y705) antibody [EP2147Y](ab76315); ab76315 staining STAT3 (phospho Y705) in MDA-MB-231 cells treated with stattic , by ICC/IF. Decrease in STAT3 (phospho Y705) expression correlates with increased concentration of stattic as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of (stattic) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76315 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.