Anti-STAT3 Antibody, EP2147Y

Also for STAT3 (NM_213662)

OriGene Data

Immunogen	A synthetic phospho-peptide corresponding to residues surrounding tyrosine 727 of human Stat3 was used as immunogen.
Clone Name	EP2147Y	Isotype	IgG
Species Reactivity	Human, Mouse	Concentration
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB: 1: 10,000; IHC: 1: 100; ICC: 1: 100; IP: 1:40
Buffer	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 3
Alternative Name	APRF; HIES
Database Link	NP_998827
Function	Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)
Related Pathway	Adipogenesis Delta-Notch Signaling Pathway EGFR1 Signaling Pathway Jak-STAT signaling pathway TGF Beta Signaling Pathway

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WB Image

Western blot analysis on HeLa cell lysates using anti-Phospho-Stat3 (pY705), 1:20,000 dilution. Cells were either (A) untreated (B) treated with IFN-α

IHC Image

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Phospho STAT3 (pY705).

IF Image

Immunofluorescent staining of HeLa cells using anti-Phospho STAT3 (pY705)

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Anti-STAT3 Antibody EP2147Y

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OriGene Data

Reference Data