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Anti-STAT3 Antibody EP2147Y
Also for STAT3 (NM_213662)
|A synthetic phospho-peptide corresponding to residues surrounding tyrosine 727 of human Stat3 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:10000 - 1:20000; IP: 1:40; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
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Western blot - STAT3 (phospho Y705) antibody [EP2147Y]; All lanes : Anti-STAT3 (phospho Y705) antibody [EP2147Y] at 1/20000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate, treated with alpha interferon.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit-HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 88 kDa.Observed band size : 75 kDa .
Western blot - Anti-STAT3 (phospho Y705) antibody [EP2147Y]; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 88 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT3 (phospho Y705) antibody [EP2147Y]; Human colon carcinoma tissue stained with TA301101 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - STAT3 (phospho Y705) antibody [EP2147Y]; HeLa cells with TA301101 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence-Anti-STAT3 (phospho Y705) antibody [EP2147Y](TA301101); TA301101 staining STAT3 (phospho Y705) in MDA-MB-231 cells treated with stattic , by ICC/IF. Decrease in STAT3 (phospho Y705) expression correlates with increased concentration of stattic as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of (stattic) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA301101 (5 ug/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.