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Anti-RELA Antibody EP2161Y
Also for RELA (NM_021975)
|A synthetic phospho-peptide corresponding to residues surrounding serine 486 of human NF-kappa-B protein. This antibody detects both phosphorylated and non-phosphorylated version of human NF-kappa-B protein|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; FC: 1:30
|Does not react with Mouse, Rat. Is unsuitable for IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IHC-P or IP.
|Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1|
|Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB. (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the I?B family retains NF-?B in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (4). A phosphorylation at serine 468 of NF-B by GSK3 beta and IKK beta has been linked negative regulation of NF-B without the inhibition of NF-B nuclear translocation after TNF-alpha or IL-1 beta stimulation (5). |
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - NFkB p65 antibody [EP2161Y]; All lanes : Anti-NF-kB p65 antibody [EP2161Y] at 1/20000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Daudi cell lysate.Lane 3 : Jurkat cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 65 kDa.Observed band size : 70 kDa .