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Anti-EIF4E Antibody EP2151Y
Also for EIF4E (NM_001968)
|A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:5000 - 1:10000; IP: 1:60; IHC-P: 1:100 - 1:250; ICC: 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1|
|AUTS19; CBP; EIF4E1; EIF4EL1; EIF4F|
|eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multi-protein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5) |
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Western blot - eIF4E (phospho S209) antibody [EP2151Y]; All lanes : Anti-eIF4E (phospho S209) antibody [EP2151Y] at 1/10000 dilution.Lane 1 : 293 cell lysates, untreated.Lane 2 : 293 cell lysates, treated with AP.Lysates/proteins at 10 µg per lane.Predicted band size : 25 kDa.Observed band size : 25 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y]; ab76256 at 1/100 dilution staining eIF4E in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence-Anti-eIF4E (phospho S209) antibody [EP2151Y](ab76256); ab76256 staining eIF4E (phospho S209) in serum starved HEK193 cells treated with CGP 57380 , by ICC/IF. Decrease in eIF4E (phospho S209) expression correlates with increased concentration of CGP 57380, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of (CGP 57380) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76256 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.