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Home Antibody All anti-EIF4E antibodies

Anti-EIF4E Antibody EP2151Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301092
  • Rabbit Monoclonal Antibody against EIF4E (Clone EP2151Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400723) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for EIF4E (NM_001968)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E was used as an immunogen.
Clone NameEP2151Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:5000 - 1:10000; IP: 1:60; IHC-P: 1:100 - 1:250; ICC: 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1
Alternative NameAUTS19; CBP; EIF4E1; EIF4EL1; EIF4F
Database LinkNP_001959
FunctioneIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multi-protein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5)
Related Pathway

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WB Image
Western blot - eIF4E (phospho S209) antibody [EP2151Y]; All lanes : Anti-eIF4E (phospho S209) antibody [EP2151Y] at 1/10000 dilution.Lane 1 : 293 cell lysates, untreated.Lane 2 : 293 cell lysates, treated with AP.Lysates/proteins at 10 µg per lane.Predicted band size : 25 kDa.Observed band size : 25 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4E (phospho S209) antibody [EP2151Y]; TA301092 at 1/100 dilution staining eIF4E in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-eIF4E (phospho S209) antibody [EP2151Y](TA301092); TA301092 staining eIF4E (phospho S209) in serum starved HEK193 cells treated with CGP 57380 , by ICC/IF. Decrease in eIF4E (phospho S209) expression correlates with increased concentration of CGP 57380, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of (CGP 57380) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA301092 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

 

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