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Anti-LCK Antibody EP2158Y


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against LCK (Clone EP2158Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401649) , 20ug Explanation
100ul $325 3-7 Days

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart


OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyrosine 505 of human LCK was used as immunogen.
Clone NameEP2158Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens LCK proto-oncogene, Src family tyrosine kinase (LCK), transcript variant 2
Alternative NameIMD22; LSK; p56lck; pp58lck; YT16
Database LinkNP_005347
Entrez Gene 3932 Human
Entrez Gene 16818 Mouse
FunctionThe human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4).
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Natural killer cell mediated cytotoxicityT cell receptor signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Lck (phospho Y505) antibody [EP2158Y]; All lanes : Anti-Lck (phospho Y505) antibody [EP2158Y] at 1/10000 dilution.Lane 1 : Jurkat cell lysates, untreated .Lane 2 : Jurkat cell lysates, treated with AP .Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 58 kDa.Observed band size : 58 kDa.Additional bands at : 61 kDa (possible isoform).


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