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Anti-PDGFRB PHOSPHO Antibody EP2135Y
Also for PDGFRB (NM_002609)
|A phospho specific peptide corresponding to residues surrounding Tyrosine 740 of human PDGF-Receptor ? was used as an immunogen. This antibody detects PDGF-Receptor ? phosphorylated at Tyrosine 740|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:2000; ICC/IF: 1:100 - 1:250
|Does not react with Human. Is unsuitable for IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens platelet-derived growth factor receptor, beta polypeptide (PDGFRB)|
|CD140B; IBGC4; IMF1; JTK12; PDGFR; PDGFR-1; PDGFR1|
|Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-? and PDGF receptor-?. PDGF receptor-? can only bind two specific PDGF isoforms, PDGF-? and PDGF-? (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Both Tyrosine 751 and 740 is required for PDGFR-? mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (4). |
Cytokine-cytokine receptor interaction
MAPK signaling pathway
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Western blot - PDGF Receptor beta (phospho Y740) antibody [EP2135Y]; All lanes : Anti-PDGF Receptor beta (phospho Y740) antibody [EP2135Y] at 1/2000 dilution.Lane 1 : NIH 3T3 un-treated.Lane 2 : NIH 3T3 treated with PDGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 124 kDa.Observed band size : 190 kDa .