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Home Antibody All anti-PTK2 antibodies

Anti-PTK2 PHOSPHO Antibody EP2160Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301077
  • Rabbit Monoclonal Antibody against PTK2 (Clone EP2160Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
IF(3)
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Also for PTK2 (NM_153831)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding Tyrosine 397 of human FAK was used as an immunogen. This antibody detects FAK phosphorylated at Y397.
Clone NameEP2160Y IsotypeTissue culture supernatant
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsICC: Use a concentration of 5 ug/ml; WB: 1:1000 - 1:2000
BufferDoes not react with Mouse. Is unsuitable for Flow Cyt,IHC-P or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 1
Alternative NameFADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71
Database LinkNP_722560
Entrez Gene 5747 Human
Entrez Gene 25614 Rat
FunctionFocal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation.
Related PathwayProtein KinaseDruggable Genome ErbB signaling pathwayChemokine signaling pathwayAxon guidanceVEGF signaling pathwayFocal adhesionLeukocyte transendothelial migrationMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - FAK (phospho Y397) antibody [EP2160Y]; All lanes : Anti-FAK (phospho Y397) antibody [EP2160Y] at 1/2000 dilution.Lane 1 : human brain tissue lysates, untreated.Lane 2 : human brain tissue lysates treated with AP.Lane 3 : rat brain tissue lysates, untreated.Lane 4 : rat brain tissue lysates treated with AP.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti rabbit. at 1/2000 dilution.Predicted band size : 119 kDa.Observed band size : 119 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-FAK (phospho Y397) antibody [EP2160Y]; ICC/IF image of TA301077 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C in PBS containing 1% BSA and 0.1% tween. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-FAK (phospho Y397) antibody [EP2160Y](TA301077); TA301077 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (in water soluble emulsion) , by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (in water soluble emulsion), as described in literature.The cells were incubated at 37°C for 5 minutes in media containing different concentrations of (anandamide (in water soluble emulsion)) in water, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA301077 (5 ug/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-FAK (phospho Y397) antibody [EP2160Y](TA301077); TA301077 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (ethanol solution) , by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (ethanol solution), as described in literature.The cells were incubated at 37°C for 10 minutes in media containing different concentrations of (anandamide (ethanol solution)) in ethanol, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA301077 (5 ug/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.

 

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