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Anti-PRKCZ PHOSPHO Antibody EP2037AY
Also for PRKCZ (NM_002744)
|A phospho specific peptide corresponding to residues surrounding Threonine 560 of human PKC zeta was used as an immunogen. This antibody detects PKC zeta phosphorylated at Threonine 560.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:100; ICC: 1:100; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens protein kinase C, zeta (PRKCZ), transcript variant 1|
|Protein kinase C (PKC), a class of serine/threonine kinases activated by Ca2+ and/or phospholipids, is involved in a variety of cellular processes such as proliferation, differentiation and secretion. PKC zeta exhibits a constitutive kinase activity which is independent of Ca2+, phosphatidylserine and diacylglycerol. Arachidonic acid alone or a combination of gamma-linolenic acid and phosphatidylserine slightly enhance PKC zeta activity. PKC zeta, in contrast to PKC alpha, is insensitive to PKC inhibitors known to interfere either with the regulatory or the catalytic domain and cannot be activated by phorbol ester treatment of NIH 3T3 cells or insect cells, overexpressing the respective PKC isoenzyme (1). Results have identified PDK-1 as the kinase that phosphorylates and activates PKC zeta in the PI 3-kinase signaling pathway. This phosphorylation and activation of PKC zeta by PDK-1 is enhanced in the presence of Ptdins-3,4-5-P3. PKCs are enzymes that are regulated at the plasma membrane, and a membrane-targeted PKC zeta is constitutively active in the absence of agonist stimulation. The association between PKC zeta and PDK-1 reveals extensive cross-talk between enzymes in the PI 3-kinase signaling pathway. (2) |
EGFR1 Signaling Pathway
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY]; All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY].Lane 1 : Hela Cell Lysate (untreated with Calyculin A).Lane 2 : Hela Cell Lysate (Treated with Calyculin A).Predicted band size : 68 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC zeta (phospho T560) antibody [EP2037AY]; ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC zeta (phospho T560) antibody [EP2037AY]; ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Flow Cytometry-Anti-PKC zeta (phospho T560) antibody [EP2037AY](ab62372); Overlay histogram showing HEK293 cells stained with ab62372 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.