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Anti-PRKCZ Phospho Antibody EP2037AY
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Also for PRKCZ (NM_002744)
|A phospho specific peptide corresponding to residues surrounding Threonine 560 of human PKC zeta was used as an immunogen. This antibody detects PKC zeta phosphorylated at Threonine 560.|
|Human, Mouse, Rat
||WB: 1:50,000~100,000; IHC: 1: 100; ICC: 1: 100; IP: 1:50
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens protein kinase C, zeta (PRKCZ), transcript variant 1|
|Protein kinase C (PKC), a class of serine/threonine kinases activated by Ca2+ and/or phospholipids, is involved in a variety of cellular processes such as proliferation, differentiation and secretion. PKC zeta exhibits a constitutive kinase activity which is independent of Ca2+, phosphatidylserine and diacylglycerol. Arachidonic acid alone or a combination of gamma-linolenic acid and phosphatidylserine slightly enhance PKC zeta activity. PKC zeta, in contrast to PKC alpha, is insensitive to PKC inhibitors known to interfere either with the regulatory or the catalytic domain and cannot be activated by phorbol ester treatment of NIH 3T3 cells or insect cells, overexpressing the respective PKC isoenzyme (1). Results have identified PDK-1 as the kinase that phosphorylates and activates PKC zeta in the PI 3-kinase signaling pathway. This phosphorylation and activation of PKC zeta by PDK-1 is enhanced in the presence of Ptdins-3,4-5-P3. PKCs are enzymes that are regulated at the plasma membrane, and a membrane-targeted PKC zeta is constitutively active in the absence of agonist stimulation. The association between PKC zeta and PDK-1 reveals extensive cross-talk between enzymes in the PI 3-kinase signaling pathway. (2) |
EGFR1 Signaling Pathway
MAPK signaling pathway
Wnt Signaling Pathway
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A. Western blot analysis on HeLa cell lysates using anti-Phospho-PKC zeta (pT560) RabMAb (TA301067), 1:100,000 dilution. Cells were either (A) untreated (B) treated with Calyculin A.
Immunohistochemical analysis of paraffin-embedded human brain using anti-PKC zeta.