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Anti-PDGFRB PHOSPHO Antibody EP2136Y
Also for PDGFRB (NM_008809)
|A phospho specific peptide corresponding to residues surrounding Tyrosine 857 of human PDGF-Receptor ? was used as an immunogen. This antibody detects PDGF-Receptor ? phosphorylated at Tyrosine 857|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500 - 1:1000
|Does not react with Rat. Is unsuitable for Flow Cyt,ICC,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Mus musculus platelet derived growth factor receptor, beta polypeptide (Pdgfrb), transcript variant 2|
|AI528809; CD140b; Pdgfr; PDGFR-1|
|Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Phosphorylation of Tyrosine 857 (pY857) in the catalytic loop of the PDGF Receptor beta has been shown to increase kinase activity. Two major isoforms exist, PDGF receptor-? and PDGF receptor-?. PDGF receptor-? can only bind two specific PDGF isoforms, PDGF-? and PDGF-? (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. |
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Western blot - PDGF Receptor beta (phospho Y857) antibody [EP2136Y]; All lanes : Anti-PDGF Receptor beta (phospho Y857) antibody [EP2136Y] at 1/1000 dilution.Lane 1 : NIH3T3 cell lysates (untreated).Lane 2 : NIH3T3 cell lysates treated with PDGF.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit, HRP labeled, at 1/2000 dilution.Predicted band size : 123 kDa.Observed band size : 190 kDa .