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Anti-SYK PHOSPHO Antibody EP575(2)Y
|A phospho specific peptide corresponding to residues surround Tyrosine 525/526 of human Syk was used as an immunogen. This antibody detects Syk phosphorylated at Tyrosine 525/526.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:5000 - 1:10000; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens spleen tyrosine kinase (SYK), transcript variant 1|
Entrez Gene 6850 Human
|Syk, a non-receptor tyrosine kinase, belongs to the Syk-Zap70 family of protein tyrosine kinase (PTK) and is involved in a wide variety of cellular functions including the pathogenesis of malignant cancer (1). Ubiquitously expressed in hematopoietic cells, Syk has been found to be an effector of B cell receptor (BCR) and functions in B & T cell lymphopoiesis. In the absence of Syk, B cell development is blocked (2). Upon binding to the BCR and to the cell surface receptor of hematopoietic cells (3), Syk is activated and phosphorylates Phospholipase C resulting in the downstream activation of ERK & JNK kinase. Syk also phosphorylates PI3-K activating the Akt pathway (4). Cross-linking of the T cell antigen receptor (TCR)-CD3 complex induces rapid tyrosine phosphorylation and then activation of Syk, which in turn phosphorylates a multitude of intracellular substrates such as Cbl (5). Substitution of Tyr-525 and Tyr-526 at the autophosphorylation site of Syk in mCD8-Syk substantially reduced the kinase activity and the binding to PLC-gamma1 SH2(C) in vitro (6). |
|Protein KinaseDruggable Genome Natural killer cell mediated cytotoxicityB cell receptor signaling pathwayFc epsilon RI signaling pathwayFc gamma R-mediated phagocytosis|
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Western blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y]; All lanes : Anti-Syk (phospho Y525) [EP575(2)Y] antibody at 1/10000 dilution.Lane 1 : untreated U937 cell lysates.Lane 2 : U937 cell lysates treated with pervanadate.Lysates/proteins at 10 ug per lane.Secondary.goat anti rabbit HRP at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 75,80 kDa .