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Anti-HSPB1 Antibody EP1724
Also for HSPB1 (NM_001540)
|A synthetic peptide corresponding to residues near the N-terminus of human Hsp 27 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500 - 1:2000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens heat shock 27kDa protein 1 (HSPB1)|
|CMT2F; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27|
|Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4). |
MAPK signaling pathway
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Western blot - Hsp27 antibody [EP1724Y] - Aminoterminal end; Anti-Hsp27 antibody [EP1724] at 1/500 dilution + HeLa cell lysate at 10 µg.Secondary.goat anti rabbit, HRP labeled, at 1/2000 dilution.Predicted band size : 23 kDa.Observed band size : 23 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hsp27 antibody [EP1724Y] - Aminoterminal end; Immunohistochemical analysis of Hsp27 expression in paraffin embedded human cervical carcinoma (left) and human breast carcinoma (right) using 1/100 ab62339