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Anti-PIN1 PHOSPHO Antibody EP1480Y
Also for PIN1 (NM_006221)
|A phospho specific peptide corresponding to residues surround Serine 16 of human Pin1 was used as an immunogen. This antibody detects Pin1 phosphorylated at Serine 16.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; ICC: 1: 100~250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1), transcript variant 1|
|Human human rotamase or peptidyl-prolyl cis-trans isomerase Pin1(PPIase) is important in protein folding, assembly and/or transport. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Pin1 function in yeast. Depletion of Pin1 from yeast or HeLa cells induces mitotic arrest, whereas HeLa cells overexpressing Pin1 arrest in the G2 phase of the cell cycle. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity (1). Pin1 is a conserved mitotic regulator essential for the G2/M transition of the eukaryotic cell cycle. Pin1 displays a preference for an acidic residue N-terminal to the isomerized proline bond due to interaction of this acidic side chain with a basic cluster. This raises the possibility of phosphorylation-mediated control of Pin1-substrate interactions in cell cycle regulation (2). |
Wnt Signaling Pathway
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Western blot - Pin1 (phospho S16) antibody [EP1480Y]; All lanes : Anti-Pin1 (phospho S16) antibody [EP1480Y] at 1/500 dilution.Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate (treated with FBS + Calyculin A).Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP conjugated at 1/2000 dilution.Predicted band size : 18 kDa.Observed band size : 18 kDa.