Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 2
Synonyms:
MSK1; MSPK1; RLPK
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Serine 360 of human MSK1 was used as immunogen. The antibody will detect MSK1 phosphorylation on Serine 360.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).
Related Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot analysis on HEK293 cell lyasate using anti-MSK1 Phospho (pS360), dilution at 1:20,000. Cells were either (A) untreated or (B) treated with AP.
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