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Anti-HOXB4 Antibody EP1919Y
Also for HOXB4 (NM_024015)
|A synthetic peptide corresponding to residues in the middle region of human HoxB4 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IP: 1:40; ICC: 1:100 - 1:250; FC: 1:20; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens homeobox B4 (HOXB4)|
|HOX-2.6; HOX2; HOX2F|
|Homebox transcription factors (Hox) are encoded by highly conserved developmental genes that are involved embryonic and early hematopoietic development. Specifically, Homeobox B4 (HoxB4) is a transcription factor encoded by HOXB4 gene and it is involved in the developmental regulation of specific positional identities on the anterior-posterior axis of cells. Like all members of HOX family, HoXB4 is abundantly expressed in primitive hematopoietic stem cell (HSC), but then decline with lineage-specific terminal differentiation (1). In HSC, stem cell self-renewal activity is regulated by USF1/2 binding to HoxB4, where binding possibly favors stem cell self-renewal instead of cell differentiation. Jun-B and Fra-1 has been linked as key mediators during cell proliferation and differentiation induced by HoxB4, which leads to increase expression of Cyclin D1 and G1 shortening (2). |
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Western blot - HOXB4 antibody [EP1919Y]; Anti-HOXB4 antibody [EP1919Y] at 1/500 dilution + K562 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 28 kDa.Observed band size : 34 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HOXB4 antibody [EP1919Y]; Immunohistochemical analysis of paraffin-embedded human placental tissue, using ab76093, at 1/100 dilution.
Flow Cytometry-Anti-HOXB4 antibody [EP1919Y](ab76093); Overlay histogram showing K562 cells stained with ab76093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.