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Anti-NOS1 Antibody EP1855Y
Also for NOS1 (NM_000620)
|A synthetic peptide corresponding to residues around serine 1417 of human nNOS protein was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:500 - 1:1000; IP: 1:20; FC: 1:30 - 1:50; IHC-P: Use at an assay dependent dilution; ICC/IF: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1|
|bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS|
|NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production. |
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Western blot - nNOS (neuronal) antibody [EP1855Y]; Anti-nNOS (neuronal) antibody [EP1855Y] at 1/1000 dilution + Mouse brain tissue lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 161 kDa.Observed band size : 161 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - nNOS (neuronal) antibody [EP1855Y]; Immunohistochemical analysis of nNOS (neuronal) in paraffin embedded human heart muscle using ab76067 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - nNOS (neuronal) antibody [EP1855Y]; ab76067 staining nNOS (neuronal) in murine sperm cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed with paraformaldehyde and blocked using 2% BSA. Samples were then incubated with undiluted ab76067. The secondary used was a FITC conjugated goat anti-rabbit IgG at a 1/400 dilution..Panel A shows the specific staining of nNOS in sperm while Panel B is the control sample treated with Rabbit IgG.
Flow Cytometry - nNOS (neuronal) antibody [EP1855Y]; Overlay histogram showing PC12 cells stained with ab76067 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.