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Home Antibody All anti-FOXO3 antibodies

Anti-FOXO3 Antibody EP1949Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300963
  • Rabbit Monoclonal Antibody against FOXO3 (Clone EP1949Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400566) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
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Also for FOXO3 (NM_001455)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human FOXO3a was used as an immunogen.
Clone NameEP1949Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:2000 - 1:5000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:50 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens forkhead box O3 (FOXO3), transcript variant 1
Alternative NameAF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A
Database LinkNP_001446
Entrez Gene 2309 Human
FunctionForkhead box O-class (FOXO) transcription factors are mammalian homologue of DAF-16, a known lifespan regulator of Caenorhabditis elegans. FOXO transcription factors, such as FOXO1, FOXO3a, and FOXO4, have been linked to the regulation of cell cycle, apoptosis, DNA repair, oxidative stress, and metabolism (1). FOXO proteins are proposed to be dual regulated by Akt-mediated phosphorylation and Skp2-mediated ubiquitination, which are usually initiated by growth factors and cytokines. FOXO proteins are also activated by stress, which induces gene expression that contributes to cell cycle arrest, implicating FOXO proteins as tumor suppressors (2). Specifically, FOXO3a (FKHR-L1) is a transcriptional activator for apoptosis in the absence of survival factors, such as in neural cell death triggered by oxidative stress. In the presence of survival factors like IGF-1, AKT1/PKB phosphorylates on Thr-32 and Ser-253. However, in the absence of survival factors, FOXO3a is dephospholyated and translocated to the nucleus inducing transcription of target genes and apoptosis (3).
Related PathwayTranscription FactorsDruggable Genome Chemokine signaling pathwayNeurotrophin signaling pathwayEndometrial cancerNon-small cell lung cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - FOXO3A antibody [EP1949Y]; Anti-FOXO3A antibody [EP1949Y] at 1/5000 dilution + Jurkat cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 71 kDa.Observed band size : 90 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - FOXO3A antibody [EP1949Y]; Ab53287 (1:50) staining human FOXO3A in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

 

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