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Anti-RDX Antibody EP1862Y
|A synthetic peptide corresponding to residues near the N-terminus of human Radixin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: Use a concentration of 5 ug/ml; WB: 1:1000 - 1:10000; IP: 1:100; ICC: 1:100 - 1:250; FC: 1:100; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens radixin (RDX), transcript variant 3|
|Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin (1). The ezrin-radixin-moesin (ERM) family of proteins crosslink actin filaments and integral membrane proteins. Radixin (encoded by Rdx) is the dominant ERM protein in the liver of wildtype mice and is concentrated at bile canalicular membranes (BCMs). Findings indicate that radixin is required for secretion of conjugated bilirubin through its support of Mrp2 localization at BCMs (2). Proteins of the ezrin-radixin-moesin family are ubiquitous constituents of the submembrane cortex, especially in epithelial cells. Radixin is thus a prominent constituent of stereocilia, where it may participate in anchoring the pointed ends of actin filaments to the membrane (3). |
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Western blot - Radixin antibody [EP1862Y]; Anti-Radixin antibody [EP1862Y] at 1/1000 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 80 kDa.Observed band size : 80 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Radixin antibody [EP1862Y]; Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence-Radixin antibody [EP1862Y](ab52495); ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Radixin antibody [EP1862Y]; Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.