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Anti-MAPK9 Antibody EP1595Y
Also for MAPK9 (NM_002752)
|A synthetic peptide corresponding to residues near the C-terminus of human JNK2 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, IP, FC
||WB: 1:1000 - 1:10000; IP: Use a concentration of 5 ug/ml; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens mitogen-activated protein kinase 9 (MAPK9), transcript variant JNK2-a2|
|JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; p54a; p54aSAPK; PRKM9; SAPK; SAPK1a|
Entrez Gene 5601 Human
Entrez Gene 26420 Mouse
Entrez Gene 50658 Rat
|JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3). |
|Protein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayWnt signaling pathwayFocal adhesionToll-like receptor signaling pathwayNOD-like receptor signaling pathwayMore Pathways >> |
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Western blot - JNK2 antibody [EP1595Y]; Anti-JNK2 antibody [EP1595Y] at 1/50000 dilution + HeLa cell lysate at 10 ug.Secondary.goat anti-rabbit-HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 48 kDa.Observed band size : 54 kDa .Additional bands at : 46 kDa (possible isoform).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JNK2 antibody [EP1595Y]; TA300934 at 1/100 dilution staining JNK2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-JNK2 antibody [EP1595Y]; ICC/IF image of TA300934 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Immunoprecipitation - Anti-JNK2 antibody [EP1595Y]; JNK2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit monoclonal to JNK2 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70Â°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA300934.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 48kDa; JNK2
Flow Cytometry-Anti-JNK2 antibody [EP1595Y](TA300934); Overlay histogram showing HeLa cells stained with TA300934 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.