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Anti-MSN Antibody EP1863Y
|A synthetic peptide corresponding to residues near the C-terminus of human Moesin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||ICC/IF: Use at an assay dependent concentration; IHC-FoFr: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IP: 1:70; ICC: 1:100 - 1:250; FC: 1:100; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens moesin (MSN)|
|Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3). |
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Western blot - Moesin antibody [EP1863Y]; Anti-Moesin antibody [EP1863Y] at 1/100000 dilution + Hela cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 68 kDa.Observed band size : 68 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Moesin antibody [EP1863Y]; Immunohistochemical staining of paraffin-embedded human tonsils using ab52490 at a 1/100 dilution.
Flow Cytometry - Moesin antibody [EP1863Y]; Overlay histogram showing HeLa cells stained with ab52490 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.