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Anti-MAPK6 Antibody EP1720Y
Also for MAPK6 (NM_002748)
|A synthetic peptide corresponding to residues near the N-terminal of human ERK3 protein was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||IP: Use at an assay dependent dilution; WB: 1:1000 - 1:10000; FC: 1:45; IHC-P: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC/IF.
|Homo sapiens mitogen-activated protein kinase 6 (MAPK6)|
|ERK3; HsT17250; p97MAPK; PRKM6|
|ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4). |
MAPK signaling pathway
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Western blot - MAPK6 antibody [EP1720Y]; Anti-MAPK6 antibody [EP1720Y] at 1/1000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 63 kDa.Observed band size : 105 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MAPK6 antibody [EP1720Y]; Ab53277 (1:50) staining human MAPK6 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.