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Home Antibody All anti-PPP1CB antibodies

Anti-PPP1CB Antibody EP1804Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300926
  • Rabbit Monoclonal Antibody against PPP1CB (Clone EP1804Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400955) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for PPP1CB (NM_002709)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on human PP1 beta was used as an immunogen.
Clone NameEP1804Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:100000 - 1:200000; IP: 1:100; FC: 1:25; IHC-P: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB), transcript variant 1
Alternative NameHEL-S-80p; PP-1B; PP1B; PP1beta; PPP1CD
Database LinkNP_002700
FunctionProtein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favoring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).
Related Pathway
Focal Adhesion

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Protein Phosphatase 1 beta antibody [EP1804Y]; Anti-Protein Phosphatase 1 beta antibody [EP1804Y] at 1/200000 dilution + Jurkat cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Protein Phosphatase 1 beta antibody [EP1804Y]; Paraffin embedded human muscle labelled with TA300926 at 1/100 - 1/250 dilution
FC Image
Flow Cytometry-Anti-Protein Phosphatase 1 beta antibody [EP1804Y](TA300926); Overlay histogram showing HeLa cells stained with TA300926 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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