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Home Antibody All anti-BECN1 antibodies

Anti-BECN1 Antibody EPR1733Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300923
  • Rabbit Monoclonal Antibody against BECN1 (Clone EPR1733Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401239) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(2)
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Also for BECN1 (NM_003766)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Beclin-1 was used as an immunogen.
Clone NameEPR1733Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:5000; IHC: 1:50; ICC: 1:50 100; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens beclin 1, autophagy related (BECN1)
Alternative NameATG6; beclin1; VPS30
Database LinkNP_003757
Entrez Gene 8678 Human
FunctionThe Beclin-1 (BECN1) gene encodes a 60-kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity and tumorigenesis. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).
Related PathwayDruggable Genome Regulation of autophagy

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Beclin 1 antibody [EPR1733Y]; Anti-Beclin 1 antibody [EPR1733Y] at 1/5000 dilution + A431 cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 52 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Beclin 1 antibody [EPR1733Y]; Ab51031 (1:50) staining human Beclin 1 in human breast tissue by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Beclin 1 antibody [EPR1733Y]; Immunofluorescent staining of HeLa cells using TA300923 (1:50).
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody [EPR1733Y]; TA300923 staining beclin1 in MCF7 cells treated with pterostilbene , by ICC/IF. Increase in beclin1 expression correlates with increased concentration of pterostilbene as described in literature.The cells were incubated at 37°C for 48h in media containing different concentrations of (pterostilbene) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300923 (5 ug/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.

 

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