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Anti-BECN1 Antibody EPR1733Y
Also for BECN1 (NM_003766)
|A synthetic peptide corresponding to residues near the C-terminus of human Beclin-1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:5000; IHC: 1:50; ICC: 1:50 100; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens beclin 1, autophagy related (BECN1)|
|ATG6; beclin1; VPS30|
|The Beclin-1 (BECN1) gene encodes a 60-kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity and tumorigenesis. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3). |
Senescence and Autophagy
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Western blot - Beclin 1 antibody [EPR1733Y]; Anti-Beclin 1 antibody [EPR1733Y] at 1/5000 dilution + A431 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 52 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Beclin 1 antibody [EPR1733Y]; Ab51031 (1:50) staining human Beclin 1 in human breast tissue by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Beclin 1 antibody [EPR1733Y]; Immunofluorescent staining of HeLa cells using ab51031 (1:50).
Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody [EPR1733Y]; ab51031 staining beclin1 in MCF7 cells treated with pterostilbene , by ICC/IF. Increase in beclin1 expression correlates with increased concentration of pterostilbene as described in literature.The cells were incubated at 37°C for 48h in media containing different concentrations of (pterostilbene) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab51031 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.