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Anti-SOD1 Antibody EP1727Y
Also for SOD1 (NM_000454)
|A synthetic peptide corresponding to residues on human SOD1 was used as an immunogen.|
|Rat, Human (Does not react with: Mouse)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:50000; FC: 1:40; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens superoxide dismutase 1, soluble (SOD1)|
|ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD|
Entrez Gene 6647 Human
Entrez Gene 24786 Rat
|The eukaryotic copper,zinc superoxide dismutases are remarkably stable dimeric proteins that maintain an intrasubunit disulfide bond in the reducing environment of the cytosol and are active under a variety of stringent denaturing conditions. The structural interplay of conserved disulfide bond and metal-site occupancy in human copper,zinc superoxide dismutase (SOD1) is of increasing interest as these post-translational modifications are known to dramatically alter the catalytic chemistry, the subcellular localization, and the susceptibility of the protein to aggregation (1). Defective SOD is linked to motor neuron death and carries implications for understanding and possible treatment of the fatal neurodegenerative disorder familial amyotrophic lateral sclerosis (FALS). A crystal structure of human SOD, along with two other SOD structures, established that all 12 observed FALS mutant sites alter conserved interactions critical to the beta-barrel fold and dimer contact, rather than catalysis (2). |
|Druggable Genome Amyotrophic lateral sclerosis (ALS)Huntington's diseasePrion diseases|
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Western blot - Superoxide Dismutase 1 antibody [EP1727Y]; Anti-Superoxide Dismutase 1 antibody [EP1727Y] at 1/50000 dilution + Jurkat cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 16 kDa.Observed band size : 18 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Superoxide Dismutase 1 antibody [EP1727Y]; Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Superoxide Dismutase 1 antibody [EP1727Y]; Immunofluorescent staining of HeLa cells using TA300916 (1:100).