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Home Antibody All anti-SOD1 antibodies

Anti-SOD1 Antibody EP1727Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300916
  • Rabbit Monoclonal Antibody against SOD1 (Clone EP1727Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400160) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
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Also for SOD1 (NM_000454)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on human SOD1 was used as an immunogen.
Clone NameEP1727Y IsotypeTissue culture supernatant
Species ReactivityHuman, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:50000; FC: 1:40; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
BufferDoes not react with Mouse. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens superoxide dismutase 1, soluble (SOD1)
Alternative NameALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD
Database LinkNP_000445
FunctionThe eukaryotic copper,zinc superoxide dismutases are remarkably stable dimeric proteins that maintain an intrasubunit disulfide bond in the reducing environment of the cytosol and are active under a variety of stringent denaturing conditions. The structural interplay of conserved disulfide bond and metal-site occupancy in human copper,zinc superoxide dismutase (SOD1) is of increasing interest as these post-translational modifications are known to dramatically alter the catalytic chemistry, the subcellular localization, and the susceptibility of the protein to aggregation (1). Defective SOD is linked to motor neuron death and carries implications for understanding and possible treatment of the fatal neurodegenerative disorder familial amyotrophic lateral sclerosis (FALS). A crystal structure of human SOD, along with two other SOD structures, established that all 12 observed FALS mutant sites alter conserved interactions critical to the beta-barrel fold and dimer contact, rather than catalysis (2).
Related Pathway
Hemostasis

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WB Image
Western blot - Superoxide Dismutase 1 antibody [EP1727Y]; Anti-Superoxide Dismutase 1 antibody [EP1727Y] at 1/50000 dilution + Jurkat cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 16 kDa.Observed band size : 18 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Superoxide Dismutase 1 antibody [EP1727Y]; Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Superoxide Dismutase 1 antibody [EP1727Y]; Immunofluorescent staining of HeLa cells using TA300916 (1:100).

 

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