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Home Antibody All anti-PRKD1 antibodies

Anti-PRKD1 Antibody EP1493Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300885
  • Rabbit Monoclonal Antibody against PRKD1 (Clone EP1493Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419136) , 20ug
100ul 325 3-7 Days
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WB(1)
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Also for PRKD1 (NM_002742)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues within human PKC mu was used as an immunogen.
Clone NameEP1493Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1: 1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein kinase D1 (PRKD1)
Alternative NamePKC-MU; PKCM; PKD; PRKCM
Database LinkNP_002733
FunctionPKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. Mutation of serines 744/748 to alanines in the activation loop of intact PKD inhibits its localization to the TGN. Moreover, anti-phospho-PKD antibody, which recognizes only the activated form of PKD, recognizes the TGN-bound PKD. Thus, activation of intact PKD is important for binding to the TGN (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).
Related Pathway
EGFR1 Signaling Pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-PKC mu antibody [EP1493Y]; All lanes : Anti-PKC mu antibody [EP1493Y] at 1/1000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with PMA.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 102 kDa.Observed band size : 105 kDa .

 

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