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Home Antibody All anti-ILK antibodies

Anti-ILK Antibody EP1593Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300878
  • Rabbit Monoclonal Antibody against ILK (Clone EP1593Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401438) , 20ug Explanation
100ul 325 In Stock
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WB(3)
IHC(1)
FC(1)
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Also for ILK (NM_004517)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near N-term of human ILK protein was used as immunogen
Clone NameEP1593Y IsotypeIgG
Species ReactivityMouse, Rat, Cow, Human, African Green Monkey ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:2000 - 1:10000; IP: 1:30; ICC: 1:100 - 1:250; FC: 1:100; IHC-P: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens integrin-linked kinase (ILK), transcript variant 1
Alternative NameHEL-S-28; ILK-1; ILK-2; P59; p59ILK
Database LinkNP_004508
FunctionIntegrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).
Related Pathway
Focal Adhesion

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Integrin linked ILK antibody [EP1593Y]; Anti-Integrin linked ILK antibody [EP1593Y] at 1/2000 dilution + COS-1 cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
WB Image
Western blot - Integrin linked ILK antibody [EP1593Y]; All lanes : Anti-Integrin linked ILK antibody [EP1593Y] at 1/2000 dilution.Lane 1 : Rat Brain.Lane 2 : Rat Heart.Lane 3 : Rat Kidney.Lane 4 : Rat Spleen.Predicted band size : 51 kDa.Exposure time : 3 minutes
WB Image
Western blot - Integrin linked ILK antibody [EP1593Y]; Anti-Integrin linked ILK antibody [EP1593Y] + PC12.Predicted band size : 51 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Integrin linked ILK antibody [EP1593Y]; Immunohistochemical analysis of paraffin-embedded human heart muscle using TA300878 at a 1/50 dilution.
FC Image
Flow Cytometry - Anti-Integrin linked ILK antibody [EP1593Y]; Overlay histogram showing HEK293 cells stained with TA300878 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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