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Home Antibody All anti-LDHB antibodies

Anti-LDHB Antibody EP1566Y

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300872 Rabbit Monoclonal Antibody against LDHB (Clone EP1566Y) 100ul $325 In Stock
LC400836 LDHB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human LDH-B was used as immunogen.
Clone NameEP1566Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:50 - 1:100; WB: 1:10000; IP: 1:100; FC: 1:50 - 1:80; IHC-P: Use at an assay dependent concentration
Predicted MW Explanation 37 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens lactate dehydrogenase B (LDHB), transcript variant 1
Alternative NameHEL-S-281; LDH-B; LDH-H; LDHBD; TRG-5
Database LinkNP_002291
Entrez Gene 3945 Human
Entrez Gene 16832 Mouse
Entrez Gene 24534 Rat
FunctionLactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).
Related PathwayDruggable Genome Glycolysis / GluconeogenesisCysteine and methionine metabolismPyruvate metabolismPropanoate metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Lactate Dehydrogenase antibody [EP1566Y]; Anti-Lactate Dehydrogenase antibody [EP1566Y] at 1/100000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.
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Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y]; Immunohistochemical analysis of paraffin-embedded human liver carcinoma using TA300872 at a 1/50 dilution.
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Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody [EP1566Y]; ICC/IF image of TA300872 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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Flow Cytometry-Lactate Dehydrogenase antibody [EP1566Y](TA300872); Overlay histogram showing HeLa cells stained with TA300872 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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