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Anti-CRADD Antibody EPR1640Y
Also for CRADD (NM_003805)
|A synthetic peptide corresponding to residues on the C-terminus of human RAIDD was used as an immunogen|
||Lot dependent; please refer to CoA along with shipment
||WB: 1: 1000; IHC: 1:50 100; ICC: 1: 100 250; FC: 1:20; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens CASP2 and RIPK1 domain containing adaptor with death domain (CRADD)|
|Apoptosis is triggered by activation of initiator caspases upon complex-mediated clustering of the inactive zymogen, as occurs in the caspase-9-activating apoptosome complex. Activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD (1). RAIDD has a dual-domain structure similar to that of FADD. It has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. RAIDD is constitutively expressed in many tissues and thus could play a role in regulating apoptosis in mammalian cells (2). In metazoan cells there exists inactive polypeptide precursors (zymogens), each composed of a prodomain, which is cleaved to activate the protease, and a large and small catalytic subunit. The coupling of these 'death' proteases to signaling pathways is probably mediated by adaptor molecules that contain protein-protein interaction motifs such as the death domain (3). |
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Western blot - RAIDD antibody [EPR1640Y]; Anti-RAIDD antibody [EPR1640Y] at 1/1000 dilution + HeLa cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 23 kDa.Observed band size : 23 kDa.
Immunohistochemistry (Paraffin-embedded sections) - RAIDD antibody [EPR1640Y]; TA300857, at a 1/50 dilution, staining Human RAIDD in heart muscle, using Immunohistochemistry, Paraffin embedded tissue.