OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PPP1CA antibodies

Anti-PPP1CA Antibody EP1511Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300850
  • Rabbit Monoclonal Antibody against PPP1CA (Clone EP1511Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400954) , 20ug
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
IF(1)
FC(1)
spacer
Also for PPP1CA (NM_002708)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on human PP1 alpha was used as an immunogen.
Clone NameEP1511Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:200000; IP: 1:100; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 1
Alternative NamePP-1A; PP1A; PP1alpha; PPP1A
Database LinkNP_002699
FunctionProtein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favouring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).
Related Pathway
Focal Adhesion

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-PPP1CA + 1CB [EP1511Y] antibody; Anti-PPP1CA + 1CB [EP1511Y] antibody at 1/200000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP antibody at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.
IHC Image
Anti-PPP1CA + 1CB [EP1511Y] antibody; Ab52619 at 1/100 dilution staining human uterus carcinoma tissue; paraffin embedded.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + 1CB [EP1511Y] antibody; ICC/IF image of TA300850 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TA300850 at 1/200 dilution overnight at +4°C. The secondary antibody (green)was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry - Anti-PPP1CA + 1CB [EP1511Y] antibody; Overlay histogram showing HeLa cells stained with TA300850 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title