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Anti-CDK5 Antibody EP762RY
Also for CDK5 (NM_004935)
|A synthetic peptide corresponding to residues near the tyrosine 15 of human Cdk5 protein.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:2000; IHC-P: Use at an assay dependent dilution
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens cyclin-dependent kinase 5 (CDK5), transcript variant 1|
Entrez Gene 1020 Human
|Cyclin-dependent protein kinase 5 (Cdk5) is a unique member of the family of cyclin-dependent kinases that is not directly involved in the progression of proliferating cells through the cell cycle (1). Distributed in most tissues and various cell lines, Cdk5 is active primarily in neuronal tissues, because of its association with neuron-specific activators such as p35 (a neural-specific regulatory subunit of Cdk5) (2). In addition to its role in muscle and neuronal differentiation, Cdk5 also contributes to the assembly, organization and stability of the axonal cytoskeleton in neurons, promotes neurite outgrowth and axon guidance in developing neurons, participates in the regulation of synaptic transmission and provides an additional pathway to cellular apoptosis (3-5). Cdk5 phosphorylates MAP1b, Tau, neurofilament proteins, caldesmon and proteins of the synaptic vesicles. Deregulation of Cdk5 has profound cytotoxic effects and is implicated in the development of neurodegenerative diseases such as Alzheimer's disease and amyotrophic lateral sclerosis. Recent studies have showed that increased kinase activity of the p35/Cdk5 complex in developing neurons is observed when Cdk5 is phosphorylated at Y15 by c-Abl tyrosine kinase; this activity is further enhanced by Cable (6). |
|Protein KinaseDruggable Genome Axon guidanceAlzheimer's disease|
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Western blot - Anti-Cdk5 (phospho Y15) antibody [EP762RY]; Lanes 1 - 2 : Pan Cdk5.Lanes 3 - 4 : Anti-Cdk5 (phospho Y15) antibody [EP762RY] at 1/2000 dilution.Lane 1 : HeLa cell lysate treated with AP.Lane 2 : HeLa cell lysate.Lane 3 : HeLa cell lysate treated with AP.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 33 kDa.Observed band size : 33 kDa.Additional bands at : 160 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk5 (phospho Y15) antibody [EP762RY]; TA300847, at 1/50 dilution, staining Cdk5 in paraffin embedded human brain tissue sections by Immunohistochemistry.