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Anti-ICAM1 Antibody EP1442Y
Also for ICAM1 (NM_000201)
|A synthetic peptide corresponding to residues near the N-terminus of human ICAM-1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:2000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250; FC: 1:100
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens intercellular adhesion molecule 1 (ICAM1)|
|BB2; CD54; P3.58|
|Antigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens. Recently, ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-1. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells and disrupt the interaction between cytotoxic T cells and target cells. ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines (1). The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry (2). Monoclonal antibodies recognize a 95 kDa cell surface glycoprotein the major human rhinovirus receptor, ICAM-1 (3). |
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Western blot - ICAM1 antibody [EP1442Y]; Anti-ICAM1 antibody [EP1442Y] at 1/2000 dilution + Raji cell lysate at 10 µg.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 58 kDa.Observed band size : 89 kDa .
Immunohistochemistry (Paraffin-embedded sections) - ICAM1 antibody [EP1442Y]; ab53013, at a 1/50 dilution, staining Human ICAM1 in Tonsil using Immunohistochemistry, Paraffin embedded tissue.
Flow Cytometry - Anti-ICAM1 antibody [EP1442Y]; Overlay histogram showing Raji cells stained with ab53013 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.