Home Antibody All anti-KRT8 antibodies
Also for KRT8 (NM_002273)
|A synthetic peptide corresponding to residues on the N-terminus of human CK-8 was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:500; IP: 1:60; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Immunogen affinity purified
|Is unsuitable for Flow Cyt.
|Homo sapiens keratin 8, type II (KRT8), transcript variant 2|
|CARD2; CK-8; CK8; CYK8; K2C8; K8; KO|
Entrez Gene 3856 Human
|Keratins, the intermediate filament proteins of epithelial cells, connect to desmosomes, the cell-cell adhesion structures at the surface membrane. Among the various intermediate filament (IF) proteins, cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells (1). Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine (2). While K8/K18 are essential for maintaining structural integrity, there is accumulating evidence indicating that they also exert non-mechanical functions. It has been reported that K8/K18-free hepatocytes from K8-null mice are more sensitive to Fas-mediated apoptosis, in line with an increased Fas density at the cell surface and an altered c-Flip regulation of the anti-apoptotic ERK1/2 signaling pathway (3). |
|Druggable Genome |
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Western blot - Cytokeratin 8 antibody; Anti-Cytokeratin 8 antibody at 1/500 dilution + HeLa cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 54 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody; TA300826 at 1/100 dilution staining Cytokeratin 8 in human gastric adenocarcinoma by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody; TA300826 at 1/100 dilution staining Cytokeratin 8 in HeLa cells by Immunofluorescence.