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Home Antibody All anti-ACLY antibodies

Anti-ACLY Antibody EP737Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300817
  • Rabbit Monoclonal Antibody against ACLY (Clone EP737Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420122) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for ACLY (NM_001096)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr447 and Ser451 of human ATP-citrate (pro-S) lyase was used as immunogen. The antibody only detects ATP-citrate (pro-S) lyase phosphorylated on Threonine 447 and Serine 451.
Clone NameEP737Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000; ICC/IF: 1:100 - 1:250; IP: 1:10 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens ATP citrate lyase (ACLY), transcript variant 1
Alternative NameACL; ATPCL; CLATP
Database LinkNP_001087
Entrez Gene 47 Human
Entrez Gene 104112 Mouse
Entrez Gene 24159 Rat
FunctionATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ATP citrate lyase antibody [EP737Y]; All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] at 1/1000 dilution.Lane 1 : (A) 3T3 cell lysate (untreated cells).Lane 2 : (B) 3T3 cell lysate (insulin treated cells).Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 122 kDa.Observed band size : 100 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]; TA300817 stained HeLa cells at 1/100-1/250 dilution.

 

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