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Anti-ACLY Antibody EP737Y
Also for ACLY (NM_001096)
|A synthetic phospho-peptide corresponding to residues surrounding Thr447 and Ser451 of human ATP-citrate (pro-S) lyase was used as immunogen. The antibody only detects ATP-citrate (pro-S) lyase phosphorylated on Threonine 447 and Serine 451.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000; ICC/IF: 1:100 - 1:250; IP: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens ATP citrate lyase (ACLY), transcript variant 1|
|ACL; ATPCL; CLATP|
|ATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2). |
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Western blot - ATP citrate lyase antibody [EP737Y]; All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] at 1/1000 dilution.Lane 1 : (A) 3T3 cell lysate (untreated cells).Lane 2 : (B) 3T3 cell lysate (insulin treated cells).Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 122 kDa.Observed band size : 100 kDa .
Immunocytochemistry/ Immunofluorescence - ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]; ab53007 stained HeLa cells at 1/100-1/250 dilution.