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Anti-ITGB2 Antibody EP1288Y
Also for ITGB2 (NM_000211)
|A synthetic phospho-peptide corresponding to residues surrounding Ser745 of human LFA-?2 was used as immunogen. The antibody only detects LFA-?2 phosphorylated on Serine 745.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:500; IP: 1:100; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) (ITGB2), transcript variant 1|
|CD18; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7|
Entrez Gene 3689 Human
Entrez Gene 16414 Mouse
Entrez Gene 309684 Rat
|In the immune system, integrins have essential roles in leukocyte trafficking and function. These include immune cell attachment to endothelial and antigen-presenting cells, cytotoxicity, and extravasation into tissues (1). Integrin adhesion receptors transduce signals that control complex cell functions which require the regulation of gene expression, such as proliferation, differentiation and survival. Their intracellular domain has no catalytic function, indicating that interaction with other transducing molecules is crucial for integrin-mediated signaling. JAB1 (Jun activation domain-binding protein 1), a coactivator of the c-Jun transcription factor, has been identified as a protein that interacts with the cytoplasmic domain of the beta2 subunit of the alphaL/beta2 integrin LFA-1 (2). Patients with leukocyte adhesion molecule (CD11/CD18, beta 2 integrins) deficiency have structural defects in the common beta subunit (CD18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections (3) |
|TransmembraneDruggable Genome Cell adhesion molecules (CAMs)Natural killer cell mediated cytotoxicityLeukocyte transendothelial migrationRegulation of actin cytoskeletonViral myocarditis|
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Western blot - CD18 (phospho S745) antibody [EP1288Y]; All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] at 1/500 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate, treated with AP.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.
Immunohistochemistry (Paraffin-embedded sections) - CD18 (phospho S745) antibody [EP1288Y]; Ab52920 (1:250) staining human CD18 in human tonsil tissue by immunohistochemistry using paraffin embedded tissue.
ICC/IF image of TA300815 stained Raw 246.7 cells. The cells were incubated with the antibody TA300815 at 1/1000 dilution overnight at 4.. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-CD18 (phospho S745) antibody; Human peripheral blood lymphocytes stained with TA300815 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Gating strategy ??peripheral blood lymphocytes.